15161 - ISTH "Advanced Microscopy in the Study of Platelet Function"

Electron microscopy (EM) has been at the forefront of the cell biology in general1, and is also an important tool in the platelet and MK research field. In this webinar I will present an overview of the current status of the different electron microscopy methods that are used in platelet and MK cell biology research. I will discuss the different preparation techniques for conventional TEM and SEM, immuno-EM, whole mount TEM, cryo-EM, and show examples of recent developments in the application of (correlative) 3D EM. 

Fluorescence microscopy is commonly used in cell biology to dissect out the structure and function of many cellular processes and is widely used in the study of platelets and megakaryocytes. However, there is a physical limit to the resolving power light microscopes can achieve which limits the extent of the spatial information we can obtain. In this webinar I will present a brief introduction to platelets and demonstrate several microscopy techniques (collectively known as super-resolution microscopy) which enable us to see beyond this limit and how we are beginning to use these modalities to study platelet biology.  

 

Accreditation Statement

This activity has been planned and implemented in accordance with the accreditation requirements and policies of the Accreditation Council for Continuing Medical Education through the joint providership of The Medical College of Wisconsin and International Society on Thrombosis and Haemostasis. The Medical College of Wisconsin is accredited by the ACCME to provide continuing medical education for physicians.
 
Designation of Credit Statement:
The Medical College of Wisconsin designates this enduring material activity for a maximum of 1.0 AMA PRA Category 1 Credit(s)™. Physicians should claim only the credit commensurate with the extent of their participation in the activity.
 
Please note: The course fee is $25.00 in addition to a $2.50 processing fee which is assessed for all electronic registrations.
 
More information and online registration:  http://www.prolibraries.com/isth/
 
 

 

Target Audience

  • Cell Biologists
  • Hematologists
  • Students interested in the application of high-resolution EM techniques, cellular ultrastructure, and immuno gold localization methods related to MK development and platelet adhesive functioning

Learning Objectives

At the conclusion of this activity, I will be able to:

  • Review current status of the different electron microscopy methods that are used in platelet and MK cell biology research
  • Compare the preparation techniques for conventional TEM and SEM, immuno-EM, whole mount TEM, and cryo-EM
  • Discuss recent developments in the application of (correlative) 3D EM
  • Illustrate several microscopy techniques (collectively known as super-resolution microscopy)
  • Describe how these modalities are beginning to allow researchers to study platelet biology
Additional information

Contact

Name: 
Cary Clark
Phone Number: 
+1 (919) 929-3807
Email: 
cary_clark@isth.org
Course summary
Available credit: 
  • 1.00 AMA PRA Category 1 Credit(s)™
    AMA PRA Category 1 Credit(s)™
  • 1.00 Hours of Participation
    Hours of Participation credit.
Course opens: 
06/17/2015
Course expires: 
06/17/2016
Cost:
$2.50
Part of: 
ISTH Enduring Material Series 2015
Planning Committee:
Yukio Ozaki
Geoff Barnes
Chris Ward
Cary Clark
Stephen Watson
 
Presenter:
Electron Microscopy - Harry F.G. Heijnen, MD
Super-Resolution Microscopy - Steven Thomas, MD 
 

  Moderator Steve Watson, University of Birmingham, United Kingdom

 

All persons in control of content have NO relevant financial relationships to disclose with the exception of the following persons:  

 

Name

Company

Role

Chris Ward, MD

Bayer

Advisory Board, Speaker

 

Pfizer, BMS

Advisory Board, Speaker

 

Boehringer-Ingelheim

Advisory Board, Speaker

 

Alexion

Advisory Board

 

Medimark

Steering Committee Member, Speaker

Geoff Barnes

BMS, Pfizer, Blue Cross Blue Shield of Michigan

Primary Investigator & Co-Investigator

Yukio Ozaki

Sysmex

Advisory Board


In accordance with the ACCME®  Standards for Commercial Support Number 6, all in control of content disclosed any relevant financial relationships. These relationships were reviewed via the MCW conflict of interest resolution process and resolved

 

Available Credit

  • 1.00 AMA PRA Category 1 Credit(s)™
    AMA PRA Category 1 Credit(s)™
  • 1.00 Hours of Participation
    Hours of Participation credit.

Price

Cost:
$2.50
Please login or register to take this course.

Required Hardware/software

To view the webcast on your computer go to https://www.isth.org/  click the Events/Webinars tab and view the ISTH 2015 Archived Webinars

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