15164 - ISTH "Platelet Proteomics and Current Techniques in Measurements of Platelet Protein Phosphorylation"
Rene Zahedi - "The Use of Platelet Proteomics in Research and Diagnostics"
Khalid Naseem – "Methods for Measurement of Protein Phosphorylation in Research and the Clinic"
Moderator: Michael Tomlinson
Accreditation Statement
Target Audience
The target audience should be biologists and physicians, working with both human and mouse platelets and interested in using state-of-the-art technology to further understand the role of proteins and their post-translational modifications in platelet regulation as well as fellows and trainees interested in new methods for examining protein phosphorylation and their potential for clinical application
Learning Objectives
At the conclusion of this activity, I will be able to:
- Explain the basics about proteomic technologies that are available as well as to study platelets and describe platelet disorders, including possibilities, limitations and pitfalls
- Identify the limitations of the current methods used for examining protein phosphorylation in cells
- Analyze how flow cytometry can be used for rapid examination of protein phosphorylation in whole cells
- Outline how the use of cell permeable dyes facilitates the multiplexing of phosphorylation events in single cells leading to high throughput phosphoanalysis
- Apply how phosphoflow can be used as a drug discovery platform
- Demonstrate how phosphoflow can be used to allow kinetic resolution of protein phosphorylation in multiple cell type within a single whole blood sample
- To examine how multiparameter phosphoflow can be used as a clinical tool
Name | Company | Role |
Chris Ward, MD | Bayer | Advisory Board, Speaker |
| Pfizer, BMS | Advisory Board, Speaker |
| Boehringer-Ingelheim | Advisory Board, Speaker |
| Alexion | Advisory Board |
| Medimark | Steering Committee Member, Speaker |
Geoff Barnes | BMS, Pfizer, Blue Cross Blue Shield of Michigan | Primary Investigator & Co-Investigator |
Yukio Ozaki | Sysmex | Advisory Board |
In accordance with the ACCME® Standards for Commercial Support Number 6, all in control of content disclosed any relevant financial relationships. These relationships were reviewed via the MCW conflict of interest resolution process and resolved
Available Credit
- 1.00 AMA PRA Category 1 Credit(s)™AMA PRA Category 1 Credit(s)™
- 1.00 Hours of ParticipationHours of Participation credit.
Price
Required Hardware/software
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