Red Cell Genotyping 2014: Clinical Solutions

Bethesda, MD US
September 17, 2014

Red cell genotyping has moved from the bench to the bedside.  This Symposium will provide an update on clinical solutions that red cell genotyping provides in transfusion medicine.  Clinical and laboratory training aspects are also featured, which accompany the current molecular immunohematology opportunities.

Accurate determination of blood group phenotypes is impossible in the setting of recent transfusion. In addition, clinically relevant phenotype information cannot be determined for patients with autoimmune hemolytic anemias.  To address these issues, transfusion medicine laboratories have adopted molecular techniques for clinical application. These techniques are performed using DNA, obtained from white blood cell among other sources, rather than red cells to circumvent the limitations of serologic testing.  It is now commonplace to use molecular techniques, which enable to predict blood group phenotypes despite recent transfusion and a positive direct antiglobulin test.  In addition, molecular testing can reveal antigen variants that are not obvious with the use of red cell phenotyping, and molecular tests can be designed to evaluate genotypes in the absence of blood group antisera.

Thus, red cell genotyping and the concept of genotype (dry)-matched transfusions provide ‘clinical solutions’ to aid in the reduction of the potential alloimmunizations and transfusion reactions, and to find potential compatible blood where serological testing cannot.


ACCME Accreditation Statement:
This activity has been planned and implemented in accordance with the accreditation requirements and policies of the Accreditation Council for Continuing Medical Education through the joint providership of The Medical College of Wisconsin and Blood Center of Wisconsin. The Medical College of Wisconsin is accredited by the ACCME to provide continuing medical education for physicians.
AMA Credit Designation Statement:
The Medical College of Wisconsin designates this Live Activity for a maximum of 6 AMA PRA Category 1 Credit(s) ™.  Physicians should claim only the credit commensurate with the extent of their participation in the activity.

Hours of Participation for Allied Health Care Professionals
The Medical College of Wisconsin designates this activity for up to 6 hours of participation for continuing education for allied health professionals.  


To Register:  visit or call 414-937-6271


Target Audience

Physicians’ and scientists involved in blood transfusions

Learning Objectives

1. Describe the categorization of RhD variants and the resolution of D typing discrepancies that genotyping can provide.

2. Identify red cell genotyping strategies to prevent alloimmunization and transfusion reactions for the chronically transfused patient with Sickle Cell disease.

3. Identify red cell genotyping strategies to prevent alloimmunization and transfusion reactions in patients with warm autoimmune hemolytic anemia.

4. Discuss patient care strategies from examples (vignettes) described during case reviews.

5. Develop an appropriate red cell genotyping teaching and competency program for the transfusion service and immunohematology laboratory staff.

6. Describe the regulatory landscape for red cell genotyping.

Additional information


Phyllis Kirchner
Phone Number: 
+1 (414) 937-6271
Course summary
Available credit: 
  • 6.00 AMA PRA Category 1 Credit(s)™
    AMA PRA Category 1 Credit(s)™
  • 6.00 Hours of Participation
    Hours of Participation credit.
Course opens: 
Course expires: 
Event starts: 
09/17/2014 - 8:00am
Event ends: 
09/17/2014 - 4:00pm
Lister Hill Center Auditorium
National Library of Medicine
8600 Rockville Pike, NIH Building 38A
Bethesda, MD 20894
United States
Planning Committee Members:
Jerome Gottschall, MD
Willy Flegel, MD          
Greg Denomme, PhD, FCSMLS(D)
Sue Johnson, MSTM, MT(ASCP)SBB
Symposium Coordinators:
Phyllis Kirchner, MSTM, MT(ASCP)SH, SBB
Karen M Byrne, MT, (ASCP), SBB
Ollie Smith
Celso Bianco MD
S Gerald Sandler MD
Tom C Abshire MD
Lilian M de Castilho PhD
Frederick V Plapp MD
Harvey G Klein MD
Annette A Ragosta RAC MT(ASCP)SBB
Ellen B Klapper MD
Dan A Waxman MD
Anne A Igbokwe MD
All persons in control of content have NO relevant financial relationships to disclose with the exception of the following persons:

Willy Flegel, MD

Blutspendedienst Baden-Wuerttemberg - Hessen



Patents for RHD Genotyping Applications


Jerome Gottschall, MD

NIH Contract – RED III

Co-Principal Investigator

Susan Johnson, MSTM, MT

Novartis, Grifols, Ortho Clinical Diagnostics


Dan Waxman, MD


Advisory Board

Ellen Klapper, MD


Advisory Board

Greg Denomme

Canadian Blood Services


Available Credit

  • 6.00 AMA PRA Category 1 Credit(s)™
    AMA PRA Category 1 Credit(s)™
  • 6.00 Hours of Participation
    Hours of Participation credit.


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